| Expression pattern: |
UP |
| Associated gene: |
EZH2, AGO2, Wnt10b, Wnt1 |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
promotes preadipocyte differentiation/adipogenesis and is associated with obesity prognosis; knockdown reduces differentiation and improves obesity-related phenotypes in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-138-5p to increase EZH2 expression (ceRNA-like regulation), involving AGO2/RISC association |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; ISH (In Situ Hybridization); RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Oil Red O Staining; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; ROC Analysis; Cohort Study; Bioinformatics Analysis |
| Clinical significance: |
circSAMD4A upregulation in adipose tissues was correlated with a poor prognosis in obese patients and may predict non-remission after bariatric surgery |
| Description: |
circSAMD4A (hsa_circ_0004846), derived from hSAMD4A, is up-regulated in visceral adipose tissue of obese patients and predominantly localized in the cytoplasm. It promotes preadipocyte differentiation by sponging miR-138-5p, thereby relieving repression of EZH2 and supporting an circSAMD4A/miR-138-5p/EZH2 regulatory axis. High circSAMD4A expression is associated with poor prognosis (non-remission) after bariatric surgery and shows diagnostic performance by ROC analysis. |
| Confidence score: |
0.8965 |