| Expression pattern: |
UP |
| Associated gene: |
SLC7A5, mTORC1 |
| Associated microRNA: |
miR-20a-5p |
| Biological function: |
promotes proliferation, migration, and invasion of NSCLC cells |
| Molecular mechanism: |
acts as a ceRNA sponging miR-20a-5p to upregulate SLC7A5 and activate mTORC1 signaling |
| Biological pathway or process: |
mTORC1 (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
High LDLRAD3 expression is associated with advanced stage (III+IV vs I+II) and lower survival rate in NSCLC patients. |
| Description: |
circRNA LDLRAD3 is up-regulated in NSCLC tissues/cell lines and promotes NSCLC cell proliferation, migration, and invasion. Mechanistically, LDLRAD3 sponges miR-20a-5p to increase SLC7A5 expression and activates the mTORC1 signaling pathway, and high LDLRAD3 expression is associated with worse patient survival. |
| Confidence score: |
0.7396 |