circRNA basic information
circBase ID: hsa_circ_0005785
Name: hsa_circ_ANAPC7
Synonym: circ_0005785
Host Gene: ANAPC7
Genomic location(hg19): chr12:110819556-110834257:-
Genomic location(hg38): chr12:110381751-110396452:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

adjacent normal tissues; tumor tissues; xenograft tumors

Cell lines:

LOVO; HCT-116; SW620; NCM460; HUVECs

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: DNMT3A
Associated microRNA: miR-7-5p
Biological function: Circ_0005785 promotes CRC cell proliferation, invasion, angiogenesis, EMT and tumor growth, and suppresses apoptosis.
Molecular mechanism: Circ_0005785 acts as a ceRNA by sponging miR-7-5p to increase DNMT3A expression, thereby aggravating CRC progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); EMT (promotes)

Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis

Clinical significance:

High circ_0005785 expression was associated with poor overall survival in CRC patients.

Description:

Circ_0005785 is up-regulated in CRC tissues and cell lines, and high expression is associated with poor overall survival. Functionally, circ_0005785 promotes CRC proliferation, invasion, angiogenesis, EMT and in vivo tumor growth while inhibiting apoptosis. Mechanistically, it sponges miR-7-5p to increase DNMT3A expression, forming a circ_0005785/miR-7-5p/DNMT3A regulatory axis.

Confidence score:

0.7892

Other information
Title:

Circ_0005785 Silencing Constrains the Functional Properties of Colorectal Cancer Cells Depending on miR-7-5p/DNMT3A Axis.

Journal: Biochemical genetics
Published: 2024
PubMed ID: 37730966
Study type:

combined biological and clinical study

Data availability: GSE126094; analyzed data sets generated during the present study are available from the corresponding author on reasonable request; supplementary material available at https://doi.org/10.1007/s10528-023-10522-6
Code availability: -