| Expression pattern: |
UP |
| Associated gene: |
PD-1 / Programmed cell death protein 1 |
| Associated microRNA: |
miR-214-5p |
| Biological function: |
circFLNA was elevated in sepsis-induced ARDS; circFLNA depletion increased CD4 + CD25 + Foxp3 + Tregs and decreased inflammatory response, including TNF-alpha, IL-1beta, and IL-6. |
| Molecular mechanism: |
circFLNA acts through a ceRNA-like circFLNA/miR-214-5p/PD-1 signaling axis, binding miR-214-5p and regulating PD-1 to modulate Tregs and inflammatory response. |
| Biological pathway or process: |
ceRNA regulation (other); immune regulation (other); inflammation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Flow Cytometry（Non-apoptosis/cycle）; ELISA; In Vivo Animal Model; H&E Staining; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Highly expressed circFLNA may be an indicator of ARDS; ROC analysis showed an AUC of 0.9600. |
| Description: |
circFLNA is up-regulated in BALF from patients with sepsis-induced ARDS and in CLP-induced ARDS mouse samples. Functionally, circFLNA depletion alleviates inflammatory responses and increases CD4 + CD25 + Foxp3 + Tregs. Mechanistically, circFLNA binds miR-214-5p and regulates the downstream target PD-1, forming a circFLNA/miR-214-5p/PD-1 axis relevant to immune regulation in ARDS. |
| Confidence score: |
0.8429 |