circRNA basic information
circBase ID: -
Name: hsa_circ_PGPEP1
Synonym: circPGPEP1
Host Gene: PGPEP1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

RCC tissue; adjacent normal renal tissue; tumor tissues; normal tissues
Cell lines:

HK-2; 786-O; ACHN; Caki1; Caki2; 769-P
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: JPT1
Associated microRNA: miR-378a-3p
Biological function: Promotes RCC cell proliferation, invasion, migration, EMT, Warburg effect, tumor growth, and distant metastasis.
Molecular mechanism: circPGPEP1 acts as a ceRNA/miRNA sponge for miR-378a-3p, thereby inducing JPT1 expression and promoting RCC malignant phenotypes.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); glycolysis (promotes); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Cohort Study; Survival Analysis
Clinical significance:

High circPGPEP1 expression was positively correlated with high histological grade and distant metastasis in RCC patients, and patients with high circPGPEP1 levels had worse survival prognosis.
Description:

circPGPEP1 is up-regulated in RCC tissues and cell lines and is associated with high histological grade, distant metastasis, and poor survival. Mechanistically, cytoplasmic circPGPEP1 sponges miR-378a-3p to increase JPT1 expression, thereby promoting RCC proliferation, invasion, migration, EMT, Warburg effect, tumor growth, and distant metastasis.
Confidence score:

0.8658
Other information
Title:

Circ-PGPEP1 augments renal cell carcinoma proliferation, Warburg effect, and distant metastasis.

Journal: Acta biochimica Polonica
Published: 2023
PubMed ID: 37722013
Study type:

combined biological and clinical study
Data availability: Data is available from the corresponding author on request.
Code availability: -