| Expression pattern: |
UP |
| Associated gene: |
RAC1 |
| Associated microRNA: |
miR-493-5p |
| Biological function: |
Promotes RCC cell proliferation, migration, invasion, and Warburg effect/aerobic glycolysis; inhibits apoptosis; associated with poor prognosis. |
| Molecular mechanism: |
Circ-CSPP1 acts as a miR-493-5p sponge to upregulate RAC1, thereby promoting RCC progression and glycolysis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis; Survival Analysis; Cohort Study |
| Clinical significance: |
High circ-CSPP1 expression is associated with distant metastasis, clinical stage, tumor stage, and poor overall survival; it may serve as a diagnostic and therapeutic target for RCC. |
| Description: |
Circ-CSPP1 is up-regulated in RCC tissues and cell lines and high expression predicts poor overall survival. Functionally, circ-CSPP1 promotes RCC cell proliferation, migration, invasion, and Warburg effect while suppressing apoptosis. Mechanistically, it acts as a ceRNA sponge for miR-493-5p to upregulate RAC1. |
| Confidence score: |
0.7408 |