circRNA basic information
circBase ID: hsa_circ_0000875
Name: hsa_circ_ZBTB7A
Synonym: circPOKE
Host Gene: ZBTB7A
Genomic location(hg19): chr19:4053968-4055245:-
Genomic location(hg38): chr19:4053970-4055247:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary BC tissue; metastatic BC tissue; adjacent noncancerous tissue; tumor tissue; normal tissue; lung tissue
Cell lines:

MDA-MB-231; HCC1806; MCF7; MCF10A; BT-549; MDA-MB-436
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: USP10, Snail, Pokemon, ZBTB7A, N-cadherin, E-cadherin, ZEB-1, ALDH1A1, KLF4, KLF5, SOX2, OTUD4, TRIM25, YBX1
Associated microRNA: -
Biological function: circPOKE acts as a metastasis suppressive circRNA in breast cancer; its overexpression inhibits migration, invasion, EMT, lung metastasis, stemness and adriamycin resistance, but does not affect proliferation.
Molecular mechanism: circPOKE directly binds USP10 and competitively disrupts USP10-Snail binding, thereby promoting Snail ubiquitination and proteasomal degradation; circPOKE can also be loaded into exosomes to suppress metastatic behavior.
Biological pathway or process:

migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); stemness (inhibits); drug resistance (inhibits); ubiquitination (promotes); other pathway/process (other)
Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; MTT; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot
Clinical significance:

circPOKE is significantly downregulated in breast cancer tissues, particularly metastatic tumors, is inversely correlated with distant metastasis and negatively associated with Snail, USP10, ALDH1A1 and N-cadherin staining; it may be a potential therapeutic target or inhibitor for BC metastasis.
Description:

circPOKE is a human ZBTB7A-derived circular RNA that is downregulated in breast cancer and more strongly reduced in metastatic disease. It suppresses breast cancer migration, invasion, EMT, stemness and metastasis by competitively binding USP10 and promoting ubiquitin-mediated degradation of Snail. circPOKE can also be secreted in exosomes, and exosome-carried circPOKE attenuates lung metastasis, supporting its potential as a therapeutic inhibitor for metastatic breast cancer.
Confidence score:

0.8729
Other information
Title:

Downregulated circPOKE promotes breast cancer metastasis through activation of the USP10-Snail axis.

Journal: Oncogene
Published: 2023
PubMed ID: 37717099
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request; TCGA; GEPIA2
Code availability: -