| Expression pattern: |
DN |
| Associated gene: |
CAPRIN1, G3BP1, c-Myc, c-Myc mRNA |
| Associated microRNA: |
- |
| Biological function: |
Suppresses HCC cell proliferation and metastasis; promotes stress granule formation; inhibits translation of c-Myc |
| Molecular mechanism: |
circVAMP3 binds CAPRIN1 (and associates with CAPRIN1-G3BP1 complex) to drive CAPRIN1 phase separation, promote stress granule formation, and suppress c-Myc translation under stress conditions. |
| Biological pathway or process: |
proliferation (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Northern Blot; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Western Blot; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
Low circVAMP3 expression levels were associated with poor overall survival (p = 0.0098) in 118 HCC patients; circVAMP3 was negatively correlated with tumor size and low levels exhibited a late tumor stage and tumor vessel invasion. |
| Description: |
circVAMP3 is significantly down-regulated in HCC tissues and low expression is associated with poor overall survival. Functionally, circVAMP3 suppresses HCC proliferation and metastasis by binding CAPRIN1, promoting CAPRIN1 phase separation and stress granule formation, thereby inhibiting c-Myc translation under stress conditions. |
| Confidence score: |
0.8781 |