circRNA basic information
circBase ID: hsa_circ_0004087
Name: hsa_circ_CDYL2
Synonym: circCDYL2
Host Gene: CDYL2
Genomic location(hg19): chr16:80718434-80719026:-
Genomic location(hg38): chr16:80684537-80685129:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; adjacent non-cancerous colorectal mucosal tissues
Cell lines:

RKO; H-RKO; N-RKO; SW620; SW480; HCT116; FHC
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: Ezrin, AKT, p-AKT
Associated microRNA: -
Biological function: promotes migration
Molecular mechanism: circCDYL2 physically binds to Ezrin, increases Ezrin protein levels, and promotes AKT phosphorylation, thereby enhancing CRC cell migration.
Biological pathway or process:

migration (promotes); PI3K/AKT (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Sanger Sequencing; divergent primers PCR; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; IHC (Immunohistochemistry); Transfection; Transwell Assay; Wound Healing Assay; Bioinformatics Analysis
Clinical significance:

CircCDYL2 expression was higher in CRC tissues with lymphatic metastasis than without lymphatic metastasis.
Description:

circCDYL2 (hsa_circ_0004087), derived from CDYL2, is up-regulated in colorectal cancer tissues and CRC cell lines. It promotes CRC cell migration by binding Ezrin, increasing Ezrin protein levels, and activating AKT phosphorylation, supporting a pro-metastatic role.
Confidence score:

0.8173
Other information
Title:

circCDYL2, Overexpressed in Highly Migratory Colorectal Cancer Cells, Promotes Migration by Binding to Ezrin.

Journal: Frontiers in oncology
Published: 2021
PubMed ID: 34485151
Study type:

combined biological and clinical study
Data availability: PRJNA732514
Code availability: -