| Expression pattern: |
UP |
| Associated gene: |
IRS4, PI3K, AKT |
| Associated microRNA: |
miR-542-3p |
| Biological function: |
promoted cell viability, proliferation, migration, and invasion and suppressed apoptosis in gastric cancer; promoted tumor growth and lung metastasis in vivo |
| Molecular mechanism: |
hsa_circ_0023409 acts as a sponge for miR-542-3p to upregulate IRS4 and activate the IRS4/PI3K/AKT pathway |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; RNase R Treatment; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high expression of hsa_circ_0023409 indicates poor prognosis in GC patients and is positively correlated with tumor size, histological grade, and TNM staging |
| Description: |
In gastric cancer, hsa_circ_0023409 is up-regulated and mainly localized in the cytoplasm. It promotes proliferation, migration/invasion and inhibits apoptosis by sponging miR-542-3p to upregulate IRS4 and activate the PI3K/AKT pathway, and its high expression is associated with worse survival. |
| Confidence score: |
0.8192 |