| Expression pattern: |
DN |
| Associated gene: |
KLF4, MMP2 |
| Associated microRNA: |
miR-1233-3p |
| Biological function: |
inhibits migration and invasion; suppresses lung metastasis; acts as a tumor suppressor |
| Molecular mechanism: |
ceRNA mechanism; transcriptional/post-transcriptional regulation of KLF4 and MMP2 via miR-1233-3p sponge activity |
| Biological pathway or process: |
metastasis (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Western Blot; Dual-Luciferase Reporter Assay; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
potential prognostic factor in breast cancer; promising independent biomarker for breast cancer diagnosis; AUC 0.8645 for ROC analysis |
| Description: |
circEHMT1 is downregulated in breast cancer tissues and acts as a tumor suppressor. It inhibits migration, invasion, and lung metastasis partly by sponging miR-1233-3p and relieving repression of KLF4, thereby reducing MMP2-related metastatic potential. |
| Confidence score: |
0.7968 |