| Expression pattern: |
UP |
| Associated gene: |
DARS, AGO2, PI3K/AKT/mTOR signaling pathway |
| Associated microRNA: |
hsa-miR-1296-5p |
| Biological function: |
Promotes LUAD cell proliferation, migration, invasion, and tumor growth in vivo. |
| Molecular mechanism: |
hsa_circ_0000519 acts as a ceRNA/miRNA sponge for hsa-miR-1296-5p, upregulates DARS expression, and activates the PI3K/AKT/mTOR signaling pathway. |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
The expression of hsa_circ_0000519 was positively correlated with T grade and TNM stage in patients with LUAD; it may be a novel biomarker and therapeutic for LUAD. |
| Description: |
hsa_circ_0000519 is upregulated in LUAD tissues and cell lines and correlates with more advanced T grade and TNM stage. It promotes LUAD proliferation, migration, invasion, and xenograft tumor growth by sponging hsa-miR-1296-5p, relieving repression of DARS, and activating the PI3K/AKT/mTOR signaling pathway. |
| Confidence score: |
0.8539 |