circRNA basic information
circBase ID: hsa_circ_0002346
Name: hsa_circ_CRIM1
Synonym: CircCRIM1
Host Gene: CRIM1
Genomic location(hg19): chr2:36623756-36669878:+
Genomic location(hg38): chr2:36396613-36442735:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

xenograft tumor tissues
Cell lines:

HEK-293T; hFOB1.19; MNNG/HOS; MG63; U2OS; 143B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: NUMB, NOTCH1, HES-1
Associated microRNA: miR146a-5p
Biological function: Acts as a tumor suppressor in osteosarcoma; suppresses proliferation, migration, invasion, cell viability, and tumor growth.
Molecular mechanism: circCRIM1 sponges miR146a-5p, increases NUMB expression, and inhibits NOTCH signaling.
Biological pathway or process:

ceRNA regulation (promotes); Notch (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

Potential diagnostic and therapeutic value in osteosarcoma.
Description:

circCRIM1 is a CRIM1-derived human circRNA that is downregulated in osteosarcoma cell lines and mainly localized in the cytoplasm. In this study, circCRIM1 acted as a tumor suppressor by sponging miR146a-5p, increasing NUMB expression, and inhibiting NOTCH signaling, thereby reducing osteosarcoma proliferation, migration, invasion, and xenograft tumor growth.
Confidence score:

0.8109
Other information
Title:

CircCRIM1 suppresses osteosarcoma progression via sponging miR146a-5p and targeting NUMB.

Journal: American journal of cancer research
Published: 2023
PubMed ID: 37693139
Study type:

biological research
Data availability: GSE96964; Supplementary Materials 1, 2, 3 and 4
Code availability: Supplementary Materials 1, 2, 3 and 4