circRNA basic information
circBase ID: hsa_circ_0001821
Name: hsa_circ_PVT1
Synonym: circPVT1
Host Gene: PVT1
Genomic location(hg19): chr8:128902834-128903244:+
Genomic location(hg38): chr8:127890588-127890998:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015759
MONDO name: B-cell non-Hodgkin lymphoma
Disease details: B-cell lymphoma
Disease DO ID:
 -
Disease MeSH ID:
 -
Disease NCIt ID:
 C3457
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tonsil specimens; normal B-cell subsets; germinal center B cells
Cell lines:

ST486; CA46; BL41; DG75; L428; L1236; KM-H2; SUPHD1; SUDHL4; SUDHL5; SUDHL10; RI-1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: -
Biological function: Supports and promotes proliferation/growth of B-cell lymphoma cells; circPVT1 knockdown inhibits growth especially in BL and some HL cells, while circPVT1 overexpression promotes growth in cell lines lacking or having low endogenous circPVT1 levels.
Molecular mechanism: Growth-supporting effect independent of miRNA binding; predominant cytoplasmic localization was observed, but AGO2-RIP did not support miRNA interaction and the precise mechanism remains unresolved.
Biological pathway or process:

proliferation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Transfection
Clinical significance:

-
Description:

circPVT1 is up-regulated in B-cell lymphoma cell lines compared with germinal center B cells. Functional knockdown and overexpression experiments indicate that circPVT1 supports lymphoma cell growth, particularly in BL and in cells with absent or low endogenous circPVT1. The study found no evidence that circPVT1 binds miRNAs in B-cell lymphoma, suggesting that its proliferation-promoting role is independent of a ceRNA mechanism.
Confidence score:

0.6471
Other information
Title:

Proliferation-promoting roles of linear and circular PVT1 are independent of their ability to bind miRNAs in B-cell lymphoma.

Journal: International journal of biological macromolecules
Published: 2023
PubMed ID: 37689284
Study type:

biological research
Data availability: All data generated or analyzed during this study are included in the manuscript and/or the supplementary materials; https://doi.org/10.1016/j.ijbiomac.2023.126744
Code availability: -