circRNA basic information
circBase ID: hsa_circ_0001777
Name: hsa_circ_ESYT2
Synonym: Circular RNA_0001777
Host Gene: ESYT2
Genomic location(hg19): chr7:158580694-158591763:-
Genomic location(hg38): chr7:158788003-158799072:-
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung adenocarcinoma tissues; para-cancer normal lung tissues; xenograft tumor tissues; exosomes
Cell lines:

BEAS-2B; A549; PC9
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: PRICKLE2, Ago2
Associated microRNA: miR-942-5p
Biological function: Suppresses lung adenocarcinoma progression; inhibits proliferation, migration, invasion, glycolysis and tumor growth; promotes apoptosis.
Molecular mechanism: Circ_0001777 acts as a miR-942-5p sponge to induce PRICKLE2 expression via a circ_0001777/miR-942-5p/PRICKLE2 ceRNA axis.
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); glycolysis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Wound Healing Assay; Transwell Assay; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Cohort Study; Survival Analysis
Clinical significance:

Low circ_0001777 expression was associated with worse prognosis; circ_0001777 might be a new prognostic marker for lung adenocarcinoma patients.
Description:

Circ_0001777 is down-regulated in lung adenocarcinoma tissues and cell lines, and low expression is associated with worse prognosis. Functionally, circ_0001777 acts as a tumor suppressor by inhibiting proliferation, migration, invasion, glycolysis and xenograft tumor growth while promoting apoptosis. Mechanistically, it sponges miR-942-5p to up-regulate PRICKLE2, forming a circ_0001777/miR-942-5p/PRICKLE2 regulatory axis; it can also be packaged into exosomes.
Confidence score:

0.8656
Other information
Title:

Circular RNA Circ_0001777 Suppresses Lung Adenocarcinoma Progression In Vitro and In Vivo.

Journal: Biochemical genetics
Published: 2023
PubMed ID: 36103059
Study type:

combined biological and clinical study
Data availability: -
Code availability: -