circRNA basic information
circBase ID: hsa_circ_0084615
Name: hsa_circ_ASPH
Synonym: hsa_circRNA_104634
Host Gene: ASPH
Genomic location(hg19): chr8:62593526-62596747:-
Genomic location(hg38): chr8:61680967-61684188:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC samples; primary IECs isolated from CRC tissues; normal tissues; normal colon tissues; CRC tissues; xenografted tumors
Cell lines:

FHC; LoVo; HT-29; SW480; SW620; THP-1-DMs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: IGF2BP2, STING, FTO
Associated microRNA: -
Biological function: promotes CRC cell proliferation, migration and invasion; facilitates tumor growth; promotes M2 macrophage polarization
Molecular mechanism: circASPH interacts with IGF2BP2 to stabilize IGF2BP2 protein, thereby enhancing IGF2BP2-dependent stabilization of m6A-modified STING mRNA and promoting exosomal STING-mediated M2 macrophage polarization
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); macrophage polarization (promotes); immune regulation (promotes); mRNA stability (promotes); ubiquitination (inhibits); other pathway/process (promotes)
Detected method:
Q
S
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; In Vivo Animal Model; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Wound Healing Assay; Transwell Assay; Colony Formation Assay; EdU Staining; Luciferase Reporter Assay; ELISA; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; RNA-seq; Microarray
Clinical significance:

circASPH may serve as a promising therapeutic target for CRC treatment
Description:

circASPH is upregulated in colorectal cancer and acts as an oncogenic circRNA. It promotes CRC cell proliferation, migration, invasion and tumor growth, while also enhancing M2 macrophage polarization through an IGF2BP2-dependent stabilization of m6A-modified STING mRNA and exosomal STING transfer.
Confidence score:

0.808
Other information
Title:

CircASPH Enhances Exosomal STING to Facilitate M2 Macrophage Polarization in Colorectal Cancer.

Journal: Inflammatory bowel diseases
Published: 2023
PubMed ID: 37624989
Study type:

combined biological and clinical study
Data availability: GSE142837; GSE126094; GSE138589; GSE126095
Code availability: -