| Expression pattern: |
UP |
| Associated gene: |
LASP1 |
| Associated microRNA: |
miR-331-3p |
| Biological function: |
promotes colon cancer cell proliferation, migration and invasion and inhibits apoptosis; attenuates the suppressive effects of sevoflurane on colon cancer malignancy; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circ-PI4KA binds miR-331-3p to upregulate LASP1 |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-PI4KA expression was associated with poor survival rate of colon cancer patients; correlated with lymph node metastasis and tumor size |
| Description: |
circ-PI4KA is up-regulated in colon cancer and functions as an oncogenic circRNA. It sponges miR-331-3p to increase LASP1, promoting proliferation/migration/invasion and reducing apoptosis, thereby counteracting sevoflurane-induced suppression of malignancy and affecting tumor growth in vivo. |
| Confidence score: |
0.786 |