| Expression pattern: |
UP |
| Associated gene: |
FOXM1, beta-catenin, CTNNB1, Ago2 |
| Associated microRNA: |
miR-1286, miR-370-3p, miR-873-5p |
| Biological function: |
circZSWIM4 promotes LUAD cell proliferation and migration, inhibits apoptosis, induces EMT, activates the Wnt/beta-catenin pathway, and facilitates LUAD progression. |
| Molecular mechanism: |
circZSWIM4 acts as a cytoplasmic ceRNA that binds miR-370-3p and miR-873-5p to up-regulate FOXM1, promote FOXM1/beta-catenin interaction and beta-catenin nuclear translocation, and activate Wnt/beta-catenin signaling; it also binds miR-1286 and may target miR-370-3p to affect CTNNB1 expression. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); EMT (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; TUNEL; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circZSWIM4 might be a promising target for LUAD treatment and may contribute to identifying a new biomarker for LUAD. |
| Description: |
circZSWIM4 is up-regulated in LUAD cell lines and functions as an oncogenic circRNA. It mainly localizes in the cytoplasm, acts as a ceRNA for miR-370-3p and miR-873-5p to regulate FOXM1 and beta-catenin, activates Wnt/beta-catenin signaling, promotes proliferation, migration and EMT, and suppresses apoptosis. The study proposes circZSWIM4 as a potential biomarker and therapeutic target for LUAD. |
| Confidence score: |
0.8233 |