| Expression pattern: |
UP |
| Associated gene: |
Bcl-2, beclin1, SLC7A11, BECN1, GPX4, LC3, p62 |
| Associated microRNA: |
miR-508-3p |
| Biological function: |
circHIPK3 promotes gastric cancer cisplatin resistance and cell proliferation, suppresses apoptosis, blocks autophagy-dependent ferroptosis, and serum exosomal circHIPK3 serves as a noninvasive indicator for evaluating cisplatin therapeutic efficacy. |
| Molecular mechanism: |
circHIPK3 acts as a sponge for miR-508-3p, upregulates Bcl-2, strengthens Bcl-2 interaction with beclin1, inhibits autophagy and beclin1/SLC7A11-mediated ferroptosis, thereby enhancing cisplatin resistance. |
| Biological pathway or process: |
chemoresistance (promotes); drug resistance (promotes); autophagy (inhibits); ferroptosis (inhibits); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
serum exosomal circHIPK3 could be a noninvasive indicator to evaluate cisplatin resistance and therapeutic efficacy in gastric cancer patients. |
| Description: |
This study shows that circHIPK3 is upregulated in cisplatin-resistant gastric cancer cells, tissues, and serum exosomes. circHIPK3 promotes cisplatin resistance by sponging miR-508-3p and regulating the Bcl-2/beclin1/SLC7A11 axis, thereby suppressing autophagy-dependent ferroptosis. Serum exosomal circHIPK3 may serve as a noninvasive indicator of cisplatin resistance and therapeutic efficacy in gastric cancer. |
| Confidence score: |
0.786 |