| Expression pattern: |
UP |
| Associated gene: |
EGFR, Akt-mTOR pathway, AGO2 |
| Associated microRNA: |
miR-1299 |
| Biological function: |
ciRS-7 inhibits starvation- or rapamycin-induced autophagy of ESCC cells. |
| Molecular mechanism: |
ciRS-7 functions as a miR-1299 sponge, relieves miR-1299-mediated repression of EGFR, and modulates EGFR/Akt/mTOR signaling to inhibit autophagy in ESCC cells. |
| Biological pathway or process: |
autophagy (inhibits); EGFR (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Transfection; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
ciRS-7 expression is abnormally increased in ESCC tissues and relates to poor clinicopathologic features of patients with ESCC. |
| Description: |
This study identifies ciRS-7/CDR1as as a functional circRNA in ESCC cells that suppresses starvation- or rapamycin-induced autophagy. Mechanistically, ciRS-7 sponges miR-1299, rescues EGFR expression and downstream Akt/mTOR phosphorylation, and thereby counteracts miR-1299-induced autophagy. |
| Confidence score: |
0.5802 |