| Expression pattern: |
UP |
| Associated gene: |
HMGB3 |
| Associated microRNA: |
miR-1294 |
| Biological function: |
Promotes PTC cell growth, proliferation, migration, invasion, EMT-related changes, and in vivo xenograft tumor development. |
| Molecular mechanism: |
circ_LDLR acts as a cytoplasmic ceRNA/molecular sponge for miR-1294, thereby relieving miR-1294-mediated suppression of HMGB3 and upregulating HMGB3 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
High circ_LDLR expression was associated with lymph node metastasis, TNM stage and tumor volume, and circ_LDLR was proposed as a valuable prognostic biomarker and therapeutic target for PTC. |
| Description: |
circ_LDLR is up-regulated in PTC tissues and cell lines and is associated with larger tumor volume, advanced TNM stage and lymph node metastasis. Functionally, circ_LDLR promotes PTC proliferation, migration, invasion, EMT-related changes and xenograft tumor growth by sponging miR-1294 and increasing HMGB3 expression. |
| Confidence score: |
0.7785 |