circRNA basic information
circBase ID: hsa_circ_0030586
Name: hsa_circ_ABCC4
Synonym: CircABCC4
Host Gene: ABCC4
Genomic location(hg19): chr13:95813442-95840796:-
Genomic location(hg38): chr13:95161188-95188542:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

fresh PCa tissues; paracancerous non-tumor tissues; tumor tissues; liver and lung tissues
Cell lines:

RWPE-1; DU145; PC-3; LNCaP; 22Rv1; VCaP
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: METTL3, IGF2BP2 protein, CCAR1 mRNA, CCAR1, Wnt/beta-catenin pathway
Associated microRNA: -
Biological function: circABCC4 promotes PCa cell proliferation, stemness, migration, invasion, tumor growth, and metastasis; circABCC4 knockdown represses these malignant phenotypes.
Molecular mechanism: METTL3-mediated m6A modification increases circABCC4 expression; m6A-modified circABCC4 recruits IGF2BP2 to CCAR1 mRNA, enhances CCAR1 mRNA stability, increases CCAR1 expression, and activates Wnt/beta-catenin target genes.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); m6A modification (other); mRNA stability (promotes)
Detected method:
Q
H
Validation methods:

Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; MeRIP / MeRIP-seq; Transfection; CCK8; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Wound Healing Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

circABCC4 is highly expressed in PCa tissues and cells; high circABCC4 expression is associated with lower overall survival, preoperative PSA level, and Gleason score, suggesting potential biomarker and therapeutic target value.
Description:

circABCC4 is up-regulated in prostate cancer tissues and cells, and high circABCC4 expression is associated with poor overall survival. METTL3-mediated m6A modification increases circABCC4 expression, and circABCC4 promotes PCa stemness, proliferation, migration, invasion, growth, and metastasis by recruiting IGF2BP2 to stabilize CCAR1 mRNA and activate Wnt/beta-catenin target genes.
Confidence score:

0.8871
Other information
Title:

m6A-modified circABCC4 promotes stemness and metastasis of prostate cancer by recruiting IGF2BP2 to increase stability of CCAR1.

Journal: Cancer gene therapy
Published: 2023
PubMed ID: 37563361
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this published article; supplementary material available at https://doi.org/10.1038/s41417-023-00650-x
Code availability: -