| Expression pattern: |
DN |
| Associated gene: |
PTEN |
| Associated microRNA: |
miR-296-3p |
| Biological function: |
Alleviates PCOS symptoms in mice; inhibits granulosa-like tumor cell proliferation/viability; promotes apoptosis; blocks cell cycle progression. |
| Molecular mechanism: |
Acts as a ceRNA to sponge miR-296-3p, thereby up-regulating PTEN (mRNA and protein) and suppressing proliferation; validated by luciferase reporter and RNA pull-down assays; anti-miR-296-3p rescue supports the axis. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Western Blot; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ELISA |
| Clinical significance: |
Reduced circPSMC3 expression in ovarian granulosa cells from PCOS patients compared with normal individuals; proposed as providing clues for future therapeutic target of PCOS. |
| Description: |
circPSMC3 is down-regulated in ovarian granulosa cells from PCOS patients. Its overexpression alleviates PCOS-like phenotypes in a DHEA-induced mouse model and suppresses granulosa-like cell proliferation while promoting apoptosis. Mechanistically, circPSMC3 sponges miR-296-3p to increase PTEN expression (circPSMC3/miR-296-3p/PTEN ceRNA axis). |
| Confidence score: |
0.7554 |