| Expression pattern: |
UP |
| Associated gene: |
SP1, Ago2 |
| Associated microRNA: |
miR-1236-3p |
| Biological function: |
promotes OS cell proliferation and growth; suppresses cell apoptosis |
| Molecular mechanism: |
circ_0000253 acts through the miR-1236-3p/SP1 molecular axis; circ_0000253 directly targets and negatively regulates miR-1236-3p, thereby positively regulating SP1 expression |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; BrdU; Annexin V/PI Flow Cytometry; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
circ_0000253 may have implications for molecular follow-up after treatment and possible treatment options for OS |
| Description: |
circ_0000253 is upregulated in human osteosarcoma tissues and cell lines. Functionally, it promotes OS cell growth and proliferation while inhibiting apoptosis. Mechanistically, circ_0000253 directly targets miR-1236-3p and positively regulates SP1 expression through the miR-1236-3p/SP1 axis. |
| Confidence score: |
0.7283 |