| Expression pattern: |
UP |
| Associated gene: |
NRAS, AGO2 |
| Associated microRNA: |
miR-145-5p, miR-151-3p |
| Biological function: |
promotes proliferation, migration, invasion and methotrexate resistance in osteosarcoma cells |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-145-5p and miR-151-3p to upregulate NRAS expression in OS cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Transwell Assay; Colony Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
High expression of hsa_circ_0000073 was associated with poor stage and poor survival of OS; higher in OS patients with an inadequate response to MTX than those with a good response. |
| Description: |
hsa_circ_0000073 is upregulated in osteosarcoma and promotes malignant phenotypes (proliferation, migration, invasion) and methotrexate resistance. It functions mainly in the cytoplasm as a ceRNA that binds miR-145-5p and miR-151-3p, thereby relieving repression of NRAS. |
| Confidence score: |
0.8595 |