| Expression pattern: |
UP |
| Associated gene: |
TXNIP, Ago2 |
| Associated microRNA: |
miR-942-5p |
| Biological function: |
Circ_0001806 aggravates LPS-induced HK2 cell injury; circ_0001806 knockdown promotes cell proliferation and suppresses apoptosis, inflammation and oxidative stress. |
| Molecular mechanism: |
Circ_0001806 acts as a sponge for miR-942-5p and positively regulates TXNIP expression, thereby modulating LPS-induced HK2 cell injury; exosomes from LPS-treated HK2 cells mediate circ_0001806 delivery. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); apoptosis (promotes); inflammation (promotes); cell cycle (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Bioinformatics Analysis |
| Clinical significance: |
Circ_0001806 might be an important biomarker and potential therapeutic target for sepsis-related AKI. |
| Description: |
Circ_0001806 is up-regulated in serum from septic AKI patients and in LPS-treated HK2 cells. It promotes LPS-induced HK2 cell injury by sponging miR-942-5p and increasing TXNIP expression, while knockdown of circ_0001806 alleviates apoptosis, inflammation and oxidative stress and improves cell viability. The study also reports that exosomal circ_0001806 is up-regulated in LPS-induced HK2 cells and may contribute to circ_0001806 delivery. |
| Confidence score: |
0.8115 |