circRNA basic information
circBase ID: hsa_circ_0000119
Name: hsa_circ_MAN1A2
Synonym: circ_0000119
Host Gene: MAN1A2
Genomic location(hg19): chr1:117944807-117984947:+
Genomic location(hg38): chr1:117402185-117442325:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
4362
Disease MeSH ID:
-
Disease NCIt ID:
C9311
Disease ICD11 ID:
1256072522
Disease OMIM ID:
603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; paraneoplastic tissues; adjacent normal tissues; tumor tissues; xenograft tumors

Cell lines:

CaSki; SiHa; Ect1/E6E7

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: PAK2, Ago2, MMP2, MMP9, Ki67
Associated microRNA: miR-433-3p
Biological function: Promotes cervical cancer cell proliferation, cell cycle progression, migration, invasion, and tumor growth; inhibits apoptosis.
Molecular mechanism: circ_0000119 acts as a sponge for miR-433-3p and regulates PAK2 expression through the circ_0000119/miR-433-3p/PAK2 axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes)

Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Cohort Study; Survival Analysis; Bioinformatics Analysis

Clinical significance:

High circ_0000119 expression is associated with significantly lower overall survival in CC patients; circ_0000119 might be a promising diagnostic and therapeutic target for CC.

Description:

circ_0000119 is up-regulated in cervical cancer tissues and cell lines, and high expression is associated with poorer overall survival. Functionally, circ_0000119 promotes CC cell proliferation, cell cycle progression, migration, invasion, and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ_0000119 sponges miR-433-3p to regulate PAK2 expression via the circ_0000119/miR-433-3p/PAK2 axis.

Confidence score:

0.8767

Other information
Title:

Circular RNA circ_0000119 promotes cervical cancer cell growth and migration via miR-433-3p/PAK2 axis.

Journal: Journal of applied genetics
Published: 2023
PubMed ID: 37540462
Study type:

combined biological and clinical study

Data availability: The data sets used and/or analyzed during the current study are available from the corresponding author on reasonable request; Supplementary information available at https:// doi. org/ 10. 1007/ s13353-023-00772-w
Code availability: -