| Expression pattern: |
UP |
| Associated gene: |
ACAT2 |
| Associated microRNA: |
miR-377-5p |
| Biological function: |
promotes BCa cell proliferation, migration, invasion, tumor growth in vivo, and cholesteryl ester accumulation |
| Molecular mechanism: |
hsa_circ_0003098 acts as a miR-377-5p sponge to upregulate ACAT2 expression, inducing intracellular cholesteryl ester accumulation and promoting malignant progression in BCa |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); lipid metabolism (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high expression is associated with advanced pathological TNM stage, higher histological grade, and poor overall survival; may serve as a potential biomarker and therapeutic target for BCa |
| Description: |
hsa_circ_0003098 is upregulated in bladder cancer tissues and cell lines, and high expression is associated with poor prognosis and aggressive clinicopathological features. It promotes proliferation, migration, invasion, tumor growth, and cholesteryl ester accumulation by sponging miR-377-5p and upregulating ACAT2. The study supports hsa_circ_0003098 as an oncogenic circRNA and a potential prognostic biomarker and therapeutic target for bladder cancer. |
| Confidence score: |
0.8931 |