| Expression pattern: |
DN |
| Associated gene: |
SERPINE1 |
| Associated microRNA: |
hsa-miR-99a-5p |
| Biological function: |
CircCNOT6L plays a protective role in ischemic stroke models by supporting astrocyte viability and proliferation and attenuating apoptotic signaling under OGD/R conditions. |
| Molecular mechanism: |
CircCNOT6L functions as a ceRNA/miRNA sponge that absorbs hsa-miR-99a-5p and regulates SERPINE1 expression, thereby modulating apoptotic signals in astrocytes after OGD/R. |
| Biological pathway or process: |
apoptosis (inhibits); proliferation (promotes); ceRNA regulation (other); HIF-1 signaling (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circCNOT6L expression was lower in IS patients and negatively associated with NIHSS score, cerebral infarct volume, and several IS risk factors, suggesting a protective factor and potential severity-associated target. |
| Description: |
CircCNOT6L/hsa_circ_0006168 is down-regulated in plasma and plasma exosomes from ischemic stroke patients and in OGD/R-exposed A172 astrocyte-like cells. The study supports a protective role in which circCNOT6L sponges hsa-miR-99a-5p to maintain SERPINE1 expression, thereby attenuating astrocyte apoptotic signaling and associating with lower IS severity. |
| Confidence score: |
0.8537 |