| Expression pattern: |
UP |
| Associated gene: |
E2F7, Ago2 |
| Associated microRNA: |
miR-383 |
| Biological function: |
Circ-CCS promotes lung cancer cell growth, colony formation, migration, invasion, metastasis-associated marker expression and tumor growth, while inhibiting apoptosis. |
| Molecular mechanism: |
Circ-CCS functions as a miR-383 sponge to negatively regulate miR-383 and thereby upregulate E2F7 expression, forming the circ-CCS/miR-383/E2F7 axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-CCS expression predicts poor prognosis and may be used as a diagnostic tool or biological target for early diagnosis and advanced treatment of lung cancer. |
| Description: |
Circ-CCS is upregulated in lung cancer tissues and cell lines and its high expression is associated with poorer 5-year overall survival. Functionally, circ-CCS promotes lung cancer cell growth, migration, invasion, metastasis-related protein expression and xenograft tumor growth while suppressing apoptosis. Mechanistically, it acts as a miR-383 sponge to relieve repression of E2F7, forming a circ-CCS/miR-383/E2F7 oncogenic axis. |
| Confidence score: |
0.8281 |