circRNA basic information
circBase ID: hsa_circ_0000326
Name: -
Synonym: Circular RNA_0000326
Host Gene: -
Genomic location(hg19): chr11:65272490-65272586:+
Genomic location(hg38): chr11:65505019-65505115:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; peritumoral tissues; subcutaneous tumor tissues
Cell lines:

T47D; MCF-7; BT549; MDA-MB-231; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: YAP1, SOX2, OCT4, Ago2
Associated microRNA: miR-9-3p
Biological function: circ_0000326 promotes BC cell growth, invasion, stemness and tumor growth in vivo, and inhibits apoptosis; circ_0000326 silencing restrains these malignant phenotypes.
Molecular mechanism: circ_0000326 functions as a miR-9-3p sponge and regulates YAP1 expression through the circ_0000326-miR-9-3p-YAP1 axis.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); invasion (promotes); apoptosis (inhibits); stemness (promotes); Hippo/YAP (promotes)
Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0000326 expression was linked to shorter overall survival and poor prognosis, and was associated with clinicopathological features including lymph node metastasis, TNM grade and stage, differentiation, PR status, and molecular subtype.
Description:

circ_0000326 is up-regulated in breast cancer tissues and cell lines and high expression predicts poor overall survival. Functionally, circ_0000326 promotes BC cell viability, invasion, stemness and xenograft tumor growth while inhibiting apoptosis. Mechanistically, it sponges miR-9-3p to relieve repression of YAP1, forming a circ_0000326-miR-9-3p-YAP1 regulatory axis.
Confidence score:

0.8349
Other information
Title:

Circular RNA_0000326 accelerates breast cancer development via modulation of the miR-9-3p/YAP1 axis.

Journal: Neoplasma
Published: 2023
PubMed ID: 37498061
Study type:

combined biological and clinical study
Data availability: GSE182471
Code availability: -