| Expression pattern: |
UP |
| Associated gene: |
NKIRAS2 mRNA, NKIRAS2, STAT4 |
| Associated microRNA: |
miR-29a-3p |
| Biological function: |
promotes NSCLC cell proliferation, invasion and in vivo tumor growth |
| Molecular mechanism: |
circ_0001955 acts as a ceRNA sponge for miR-29a-3p, positively regulates NKIRAS2, and activates the NF-kappaB signaling pathway; STAT4 transcriptionally activates circ_0001955 |
| Biological pathway or process: |
NF-kappaB (promotes); proliferation (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; Co-IP; IF (Immunofluorescence); ISH (In Situ Hybridization); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0001955 is upregulated in NSCLC cells and promotes NSCLC cell proliferation, invasion, and xenograft tumor growth. Mechanistically, circ_0001955 mainly localizes to the cytoplasm and acts as a miR-29a-3p sponge to increase NKIRAS2 expression, thereby activating the NF-kappaB pathway; STAT4 was also reported to transcriptionally induce circ_0001955 upregulation. |
| Confidence score: |
0.7123 |