| Expression pattern: |
DN |
| Associated gene: |
DUSP5 |
| Associated microRNA: |
miR-135b-5p |
| Biological function: |
Circ_0000471 suppresses OC cell proliferation, migration, invasion, motility, and xenograft tumor growth, and promotes apoptosis. |
| Molecular mechanism: |
Circ_0000471 acts as a ceRNA/molecular sponge for miR-135b-5p, thereby up-regulating DUSP5 and suppressing OC progression. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Circ_0000471 expression was decreased in OC tissues and showed diagnostic value with an AUC of 0.693; the circ_0000471/miR-135b-5p/DUSP5 axis may offer novel therapeutic targets for OC patients. |
| Description: |
Circ_0000471 is down-regulated in ovarian cancer tissues and cell lines and acts as a tumor suppressor. Its overexpression inhibits proliferation, migration, invasion, motility, and xenograft tumor growth while promoting apoptosis, mainly through sponging miR-135b-5p to increase DUSP5 expression. The study also suggests potential diagnostic value for circ_0000471 expression in OC patients. |
| Confidence score: |
0.8769 |