| Expression pattern: |
UP |
| Associated gene: |
KDM2A |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
circGOLPH3 promotes OSCC cell growth, proliferation, migration, invasion, EMT, glycolysis and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circGOLPH3 acts as a cytoplasmic ceRNA sponge for miR-145-5p, thereby competitively inhibiting miR-145-5p-mediated repression of KDM2A and promoting OSCC malignant behaviors and glycolysis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Western Blot; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circGOLPH3 was elevated in OSCC tissues and correlated with TNM staging; it may be a new target for OSCC pharmacologic therapy. |
| Description: |
circGOLPH3 is upregulated in OSCC tissues and cell lines and is associated with higher tumor stage. Functionally, circGOLPH3 acts as a pro-oncogenic circRNA that promotes OSCC proliferation, migration, invasion, EMT, glycolysis and tumor growth while suppressing apoptosis. Mechanistically, cytoplasmic circGOLPH3 sponges miR-145-5p to increase KDM2A expression through a ceRNA mechanism. |
| Confidence score: |
0.8174 |