| Expression pattern: |
DN |
| Associated gene: |
MCL1, METTL3, FTO |
| Associated microRNA: |
miR-181a-5p |
| Biological function: |
promotes trophoblast proliferation and invasion, and inhibits apoptosis |
| Molecular mechanism: |
m6A-modified circSETD2 acts as a ceRNA/miRNA sponge to suppress miR-181a-5p and increase MCL1 transcription |
| Biological pathway or process: |
apoptosis (inhibits); proliferation (promotes); invasion (promotes); ceRNA regulation (promotes); m6A modification (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; In Vivo Animal Model; TUNEL; Transwell Assay; CCK8; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Bioinformatics Analysis |
| Clinical significance: |
circSETD2 may be involved in the pathogenesis of PE and may serve as a potential biomarker/therapeutic target for trophoblast dysfunction in PE |
| Description: |
circSETD2 is down-regulated in preeclampsia-associated trophoblasts and its overexpression promotes trophoblast proliferation and invasion while inhibiting apoptosis. Mechanistically, m6A modification upregulates circSETD2, which acts as a ceRNA sponge for miR-181a-5p to increase MCL1 transcription and thereby protect trophoblasts from apoptosis. |
| Confidence score: |
0.6253 |