circRNA basic information
circBase ID: hsa_circ_0001791
Name: hsa_circ_ADAM9
Synonym: circ-ADAM9
Host Gene: ADAM9
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; adjacent noncancerous tissues; tumor tissues
Cell lines:

MCF-7; MDA-MB-468; MDA-MB-231; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PFN2, Ago2
Associated microRNA: miR-383-5p
Biological function: circ-ADAM9 promotes proliferation, migration, invasion, tumor growth and radioresistance, while inhibiting apoptosis in breast cancer cells; circ-ADAM9 knockdown impairs malignant phenotypes and enhances radiosensitivity.
Molecular mechanism: circ-ADAM9 acts as a competing endogenous RNA by sponging miR-383-5p, thereby increasing PFN2 expression and promoting breast cancer progression and radioresistance.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); radioresistance (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; Colony Formation Assay; MTT; Annexin V/PI Flow Cytometry; Western Blot; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Bioinformatics Analysis
Clinical significance:

-
Description:

circ-ADAM9 is up-regulated in breast cancer tissues and cells. It promotes malignant phenotypes and radioresistance by sponging miR-383-5p and increasing PFN2 expression; knockdown of circ-ADAM9 suppresses proliferation, migration, invasion and tumor growth while enhancing apoptosis and radiosensitivity.
Confidence score:

0.8119
Other information
Title:

Knockdown of circ-ADAM9 inhibits malignant phenotype and enhances radiosensitivity in breast cancer cells via acting as a sponge for miR-383-5p.

Journal: Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]
Published: 2023
PubMed ID: 36205752
Study type:

combined biological and clinical study
Data availability: -
Code availability: -