circRNA basic information
circBase ID: -
Name: hsa_circ_PVT1
Synonym: circular RNA PVT1 / circPVT1
Host Gene: PVT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0018874
MONDO name: acute myeloid leukemia
Disease details: acute myeloid leukemia / AML
Disease DO ID:
9119
Disease MeSH ID:
D015470
Disease NCIt ID:
C3171
Disease ICD11 ID:
-
Disease OMIM ID:
601626
Species: Human
Species details: Homo sapiens
Tissue specimen:

bone marrow mononuclear cells (BMMCs)

Cell lines:

THP-1; KG-1a; HL-60

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: c-Myc, CXCR4
Associated microRNA: -
Biological function: Promotes proliferation and migration and inhibits apoptosis in AML cells.
Molecular mechanism: Stabilizes c-Myc protein by reducing phosphorylation at Thr-58, thereby promoting downstream CXCR4 expression; c-Myc overexpression partially rescues circPVT1-knockdown phenotypes.
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); other pathway/process (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; Survival Analysis

Clinical significance:

Higher expression of circPVT1 was associated with shorter overall survival (OS) and relapse-free survival (RFS) in AML patients.

Description:

circPVT1 is up-regulated in AML bone marrow samples and AML cell lines and its high expression is associated with shorter OS and RFS. Functionally, circPVT1 promotes THP-1 cell proliferation and migration and inhibits apoptosis. Mechanistically, circPVT1 stabilizes c-Myc protein (via reduced Thr-58 phosphorylation) and enhances downstream CXCR4 expression, and c-Myc overexpression partially rescues circPVT1 knockdown effects.

Confidence score:

0.6898

Other information
Title:

Circular RNA PVT1 Regulates Cell Proliferation, Migration, and Apoptosis by Stabilizing c-Myc and Downstream Target CXCR4 Expression in Acute Myeloid Leukemia.

Journal: Turkish journal of haematology : official journal of Turkish Society of Haematology
Published: 2023
PubMed ID: 36718632
Study type:

combined biological and clinical study

Data availability: -
Code availability: -