| Expression pattern: |
UP |
| Associated gene: |
STAT3, JAK/STAT3 signaling pathway, Bcl-2 |
| Associated microRNA: |
miR-23a-3p, miR-625-5p |
| Biological function: |
Promotes OSCC cell proliferation and inhibits apoptosis; circ_0005050 knockdown restricts proliferation and facilitates apoptosis. |
| Molecular mechanism: |
circ_0005050 functions through a ceRNA mechanism by competitively binding miR-23a-3p and miR-625-5p, up-regulating STAT3 and activating the JAK/STAT3 signaling pathway. |
| Biological pathway or process: |
JAK/STAT (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; EdU Staining; CCK8; Annexin V/PI Flow Cytometry; TUNEL; Transwell Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential diagnostic biomarker for OSCC, although the study lacked clinical samples. |
| Description: |
circ_0005050 is up-regulated in OSCC cell lines and promotes malignant growth by enhancing proliferation and suppressing apoptosis. Mechanistically, it sponges miR-23a-3p and miR-625-5p to increase STAT3 expression and activate the JAK/STAT3 signaling pathway. |
| Confidence score: |
0.7624 |