| Expression pattern: |
UP |
| Associated gene: |
SRSF3, PDCD4, PDCD4 mRNA, HSPA2, ERK1/2, p-ERK, MMP-2, LYVE-1, ICAM-1 |
| Associated microRNA: |
- |
| Biological function: |
Promotes GC cell proliferation, invasion, migration and tumor growth; inhibits apoptosis; promotes HLEC migration, invasion and tube formation; promotes lymphangiogenesis and lymph node metastasis. |
| Molecular mechanism: |
Protein interaction mechanism: hsa_circ_0000437 binds SRSF3 to suppress PDCD4 expression and promote GC growth; exosomal hsa_circ_0000437 binds HSPA2 and activates ERK signaling in HLECs independently of VEGF-C to promote lymphangiogenesis and LNM. |
| Biological pathway or process: |
ERK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); angiogenesis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; circRNA-seq; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Serum exosomal hsa_circ_0000437 is enriched in GC patients, associated with LNM, T stage, N stage and differentiation, discriminates GC patients from healthy controls and GC patients with LNM from those without LNM, and high serum exosomal hsa_circ_0000437 is associated with poor survival outcomes. |
| Description: |
hsa_circ_0000437 is a CORO1C-derived circRNA upregulated in gastric cancer tissues, cells and serum exosomes. It promotes GC tumorigenic phenotypes through the SRSF3/PDCD4 axis and promotes lymphangiogenesis and lymph node metastasis via exosomal transfer to HLECs and HSPA2-ERK signaling. Clinically, high serum exosomal hsa_circ_0000437 is associated with LNM and poor survival and may serve as a diagnostic/prognostic biomarker and therapeutic target. |
| Confidence score: |
0.8824 |