| Expression pattern: |
DN |
| Associated gene: |
FBXW7 |
| Associated microRNA: |
miR-527 |
| Biological function: |
inhibits HCC cell proliferation and invasion; tumor suppressor in HCC |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0001306 sponges miR-527 to reduce its inhibitory effect on FBXW7, thereby affecting HCC progression |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); invasion (inhibits); cell cycle (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; In Vivo Animal Model; Western Blot; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low expression level of hsa_circ_0001306 was associated with a larger tumor size of HCC; overall survival time between the high and low expression groups was not different |
| Description: |
hsa_circ_0001306 is down-regulated in hepatocellular carcinoma and functions as a tumor suppressor. It mainly acts as a ceRNA that sponges miR-527 to relieve repression of FBXW7, thereby inhibiting HCC proliferation, invasion and cell-cycle progression; knockdown enhances xenograft tumor growth. |
| Confidence score: |
0.8256 |