| Expression pattern: |
DN |
| Associated gene: |
CELF2, PTEN, AKT |
| Associated microRNA: |
miR-429 |
| Biological function: |
inhibits proliferation, migration and invasion; promotes apoptosis and cell-cycle arrest; suppresses tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism; acts as a sponge of miR-429 to regulate CELF2 and PTEN/AKT-signaling pathways |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (promotes); PI3K/AKT (inhibits); PTEN/AKT (inhibits); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Microarray |
| Clinical significance: |
circLIFR was closely connected with the clinical stage and tumor metastasis of NSCLC patients; circLIFR could play a potential diagnostics or prognostic biomarker for patients with NSCLC. |
| Description: |
circLIFR is downregulated in NSCLC and acts as a tumor suppressor. It functions as a miR-429 sponge to upregulate CELF2 and activate PTEN-related suppression of AKT signaling, thereby inhibiting proliferation, migration, invasion, and tumor growth while promoting apoptosis and cell-cycle arrest. |
| Confidence score: |
0.7769 |