| Expression pattern: |
DN |
| Associated gene: |
PLCD3 |
| Associated microRNA: |
miR-193a-3p |
| Biological function: |
CircMAP3K4 overexpression protected SRA01/04 human lens epithelial cells from H2O2-induced dysfunction by promoting cell viability and proliferation and inhibiting senescence, apoptosis, and oxidative stress. |
| Molecular mechanism: |
CircMAP3K4 acts as a molecular sponge for miR-193a-3p and up-regulates PLCD3 expression through the miR-193a-3p/PLCD3 axis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
CircMAP3K4 was down-regulated in ARC patient lens epithelium tissues and was proposed as a potential target for prevention and therapy of ARC. |
| Description: |
CircMAP3K4 (hsa_circ_0078619) is down-regulated in age-related cataract patient lens epithelium tissues and in H2O2-induced SRA01/04 human lens epithelial cells. Its overexpression protects cells from H2O2-induced dysfunction by promoting viability and proliferation while inhibiting senescence, apoptosis, and oxidative stress. Mechanistically, circMAP3K4 functions as a miR-193a-3p sponge to up-regulate PLCD3 in the circMAP3K4/miR-193a-3p/PLCD3 axis. |
| Confidence score: |
0.8107 |