| Expression pattern: |
UP |
| Associated gene: |
4EBP-1, HIF-1alpha, eIF4G |
| Associated microRNA: |
miR-125a-5p, miR-138-5p |
| Biological function: |
promotes proliferation, migration, invasion, tumor growth, angiogenesis and metastasis in colorectal cancer |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-125a-5p and miR-138-5p to increase 4EBP-1, promoting cap-independent translation of HIF-1alpha and activating HIF-1alpha signaling. |
| Biological pathway or process: |
HIF-1 signaling (promotes); angiogenesis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot; Co-IP; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circ-ERBIN was positively associated with more aggressive characterization; higher in stage III/IV than stage I/II; higher in CRC tissues than surrounding tissues |
| Description: |
circ-ERBIN is up-regulated in colorectal cancer and promotes proliferation, migration/invasion, angiogenesis, and metastasis. It functions as a ceRNA sponging miR-125a-5p and miR-138-5p to increase 4EBP-1, thereby enhancing cap-independent translation of HIF-1alpha and activating HIF-1 signaling. |
| Confidence score: |
0.8635 |