| Expression pattern: |
DN |
| Associated gene: |
HDAC4 |
| Associated microRNA: |
miR-495-3p |
| Biological function: |
circ_HIPK3 overexpression inhibits H2O2-induced lens epithelial cell apoptosis and fibrosis, promotes proliferation/viability, and alleviates lens epithelial cell injury in age-related cataract models. |
| Molecular mechanism: |
circ_HIPK3 acts as a sponge for miR-495-3p and positively regulates HDAC4 through the circ_HIPK3/miR-495-3p/HDAC4 axis. |
| Biological pathway or process: |
ceRNA regulation (other); apoptosis (inhibits); fibrosis (inhibits); proliferation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Western Blot; MTT; Annexin V/PI Flow Cytometry |
| Clinical significance: |
Targeted promotion of circ_HIPK3 expression may be a potential way to treat ARC and may provide a potential target for ARC treatment. |
| Description: |
circ_HIPK3 is down-regulated in ARC tissues and H2O2-induced human lens epithelial SRA01/04 cells. Functionally, circ_HIPK3 overexpression alleviates H2O2-induced lens epithelial cell injury by promoting proliferation and suppressing apoptosis and fibrosis. Mechanistically, circ_HIPK3 sponges miR-495-3p to positively regulate HDAC4, forming a circ_HIPK3/miR-495-3p/HDAC4 axis with potential therapeutic relevance for ARC. |
| Confidence score: |
0.6479 |