| Expression pattern: |
UP |
| Associated gene: |
RRM2 |
| Associated microRNA: |
miR-384 |
| Biological function: |
Promotes HCC cell proliferation, migration, invasion, EMT and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ_0008285 acts as a miR-384 sponge to regulate RRM2 expression through a circ_0008285/miR-384/RRM2 ceRNA axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_0008285 has potential as a marker for diagnosis and treatment and as a therapeutic target for HCC. |
| Description: |
circ_0008285 is up-regulated in HCC tissues and cell lines and functions as an oncogenic circRNA. Its knockdown suppresses proliferation, migration, invasion, EMT and xenograft tumor growth while promoting apoptosis, mainly through sponging miR-384 to increase RRM2 expression. The study suggests circ_0008285 may be a diagnostic/treatment marker and therapeutic target for HCC. |
| Confidence score: |
0.73 |