| Expression pattern: |
UP |
| Associated gene: |
HMGA2, Ras, ERK1/2, PCNA, HK2, OCT4 |
| Associated microRNA: |
miR-217 |
| Biological function: |
Promotes HCC cell proliferation/viability, glycolysis and stemness; promotes tumor growth; induces less apoptosis and less G2/M arrest (knockdown increases apoptosis and G2/M arrest). |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-217 to upregulate HMGA2, affecting downstream proliferation/glycolysis/stemness markers (e.g., PCNA, HK2, OCT4; Ras/ERK). |
| Biological pathway or process: |
glycolysis (promotes); stemness (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (other); ERK (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Colony Formation Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; RNA-seq |
| Clinical significance: |
Upregulation of circBNC2 was related to poor prognosis and tumor node metastasis (TNM) stage. |
| Description: |
circBNC2 is up-regulated in HCC tissues/cells and is associated with worse survival and more aggressive clinicopathological features. Functionally, circBNC2 promotes HCC proliferation, glycolysis and stemness and supports tumor growth in xenografts. Mechanistically, it acts as a ceRNA sponge for miR-217 to de-repress HMGA2, influencing downstream growth/glycolysis/stemness markers such as PCNA, HK2 and OCT4. |
| Confidence score: |
0.8354 |