circRNA basic information
circBase ID: hsa_circ_0008012
Name: hsa_circ_NFKB1
Synonym: circP50
Host Gene: -
Genomic location(hg19): chr4:103446668-103459113:+
Genomic location(hg38): chr4:102525511-102537956:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

A549
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: p53, p21, PUMA, Caspase3, Bcl-xS, IKB-alpha, p65, p50, C-myc
Associated microRNA: -
Biological function: circP50 mediates inorganic arsenic-induced apoptosis and inhibits proliferation of A549 cells; knockdown of circP50 promotes A549 cell viability and proliferation.
Molecular mechanism: circP50 mainly functions through the phosphorylation- and acetylation-activated p53 pathway; circP50 knockdown inhibits p53 phosphorylation and acetylation and decreases downstream pro-apoptotic proteins including p21, PUMA, Caspase3, and Bcl-xS.
Biological pathway or process:

p53 signaling (promotes); apoptosis (promotes); proliferation (inhibits); NF-kappaB (other)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; EdU Staining; Western Blot
Clinical significance:

-
Description:

This study reports that circP50/hsa_circ_0008012 is up-regulated in A549 lung adenocarcinoma cells after inorganic arsenic exposure. Functional knockdown experiments indicate that circP50 suppresses A549 cell proliferation and supports arsenic-induced apoptosis mainly through activation of p53 phosphorylation and acetylation and downstream p53 target genes.
Confidence score:

0.414
Other information
Title:

CircP50 functions through the phosphorylation- and acetylation-activated p53 pathway to mediate inorganic arsenic-induced apoptosis in A549 cells.

Journal: Environmental science and pollution research international
Published: 2022
PubMed ID: 35881289
Study type:

biological research
Data availability: All data generated or analyzed during this study are included in this published article.
Code availability: -