| Expression pattern: |
UP |
| Associated gene: |
DHX9, ADAR1, QKI, hif1a, hif2a |
| Associated microRNA: |
miR-216, miR-128, miR-143, miR-149, miR-154, miR-485 |
| Biological function: |
promotes proliferation; reduces apoptosis; promotes cisplatin resistance; promotes tumor growth in vivo; promotes stem-like properties (self-renewal/sphere formation) |
| Molecular mechanism: |
CircELP3 is elevated by hypoxia independently of HIF-related pathway and host gene (ELP3) expression changes; it is mainly cytoplasmic and is suggested to act via ceRNA activity as a miRNA sponge (predicted). |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); chemoresistance (promotes); stemness (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
High circELP3 level is correlated with advanced tumor stage (T stage > T2) and lymph node metastasis; circELP3 is upregulated in bladder cancer tissues versus adjacent normal tissues. |
| Description: |
circELP3 is upregulated in bladder cancer tissues and is induced by hypoxia in bladder cancer cell lines. Functionally, circELP3 promotes proliferation, reduces apoptosis, enhances cisplatin resistance, and supports stem-like properties; circELP3 knockdown suppresses tumor growth in a nude mouse xenograft model. It localizes mainly to the cytoplasm and is suggested to act via a ceRNA/miRNA-sponge mechanism (predicted). |
| Confidence score: |
0.6956 |