| Expression pattern: |
UP |
| Associated gene: |
VEGFA, Ago2 |
| Associated microRNA: |
miR-374b-3p |
| Biological function: |
promotes ccRCC cell proliferation and angiogenesis; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-374b-3p to upregulate VEGFA and activate Ras/ERK signaling |
| Biological pathway or process: |
proliferation (promotes); angiogenesis (promotes); ERK (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; ISH (In Situ Hybridization); FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Tube Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Clinical Sample Validation; Cohort Study; Bioinformatics Analysis |
| Clinical significance: |
High circAFAP1 level was linked to shorter overall survival and could serve as a prognostic marker in ccRCC |
| Description: |
circAFAP1 is up-regulated in ccRCC and promotes tumor cell proliferation and angiogenesis. It functions as a ceRNA by sponging miR-374b-3p to increase VEGFA expression and activate Ras/ERK signaling, and higher circAFAP1 is associated with worse overall survival. |
| Confidence score: |
0.8847 |