| Expression pattern: |
UP |
| Associated gene: |
MMP13 |
| Associated microRNA: |
miR-636, miR-665, miR-217, miR-646, miR-136 |
| Biological function: |
promotes chondrocyte ECM degradation; regulates MMP13 expression; decreases ECM formation |
| Molecular mechanism: |
Functions as a miR-136 sponge (ceRNA) to regulate MMP13 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
potential target in OA therapy |
| Description: |
circRNA-CER (circRNA_100876; hsa_circ_0023404; host gene RNF121) is up-regulated in osteoarthritis cartilage and in cytokine-stimulated chondrocytes. It promotes cartilage ECM degradation by acting as a ceRNA that sponges miR-136, thereby regulating MMP13 expression; knockdown reduces MMP13 and increases ECM markers (COL2 and aggrecan). |
| Confidence score: |
0.6676 |