| Expression pattern: |
UP |
| Associated gene: |
CCL2, Ago2 |
| Associated microRNA: |
let-7a-2-3p |
| Biological function: |
Promotes LUAD cell proliferation, migration, invasion, EMT progression, tumor growth, TAM M2 polarization and infiltration; inhibits apoptosis. |
| Molecular mechanism: |
circHSPB6 acts as a ceRNA by sponging let-7a-2-3p to regulate CCL2 expression, thereby promoting TAM M2 polarization and infiltration and accelerating LUAD malignant phenotypes. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); metastasis (promotes); macrophage polarization (promotes); immune regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Bioinformatics Analysis |
| Clinical significance: |
Potential targeted therapy and molecular theoretical basis for clinical administration in LUAD. |
| Description: |
circHSPB6/has_circ_0050648 is up-regulated in LUAD tissues and cells and functions as an oncogenic circRNA. It sponges let-7a-2-3p to regulate CCL2, promoting TAM M2 polarization and infiltration, LUAD cell proliferation, migration, invasion and EMT, while suppressing apoptosis. Knockdown of circHSPB6 inhibits malignant phenotypes in vitro and tumor growth in a cell line-derived xenograft model. |
| Confidence score: |
0.7898 |