| Expression pattern: |
UP |
| Associated gene: |
ZEB1 mRNA, AGO2 |
| Associated microRNA: |
miR-431 |
| Biological function: |
circMAT2B promotes NSCLC cell proliferation, colony formation, migration, invasion and EMT; circMAT2B knockdown inhibits these malignant traits. |
| Molecular mechanism: |
circMAT2B functions as a ceRNA by directly binding and sponging miR-431, negatively regulating miR-431 expression and promoting ZEB1-associated EMT in NSCLC cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circMAT2B expression is significantly correlated with large tumor size, advanced TNM stage and poor overall survival of NSCLC patients; circMAT2B could serve as a promising therapeutic target. |
| Description: |
circMAT2B is up-regulated in NSCLC tissues and cell lines and high expression is associated with larger tumor size, advanced TNM stage and poor overall survival. Functionally, circMAT2B promotes NSCLC cell proliferation, migration, invasion and EMT. Mechanistically, it acts as a cytoplasmic ceRNA by sponging miR-431 and influencing the miR-431/ZEB1 EMT-related axis. |
| Confidence score: |
0.7548 |