circRNA basic information
circBase ID: hsa_circ_0096157
Name: hsa_circ_MALAT1
Synonym: -
Host Gene: MALAT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small-cell lung carcinoma
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

A549; A549/DDP
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: P21, E-cadherin, CDK4, Cyclin D1, Bcl-2, N-cadherin, Vimentin
Associated microRNA: -
Biological function: Increased hsa_circ_0096157 contributed to Cisplatin resistance in NSCLC cells by promoting proliferation, migration, invasion and cell cycle progression and by suppressing apoptosis.
Molecular mechanism: hsa_circ_0096157 regulates cell cycle signaling and EMT-associated proteins, including P21, CDK4, Cyclin D1, Bcl-2, E-cadherin, N-cadherin, and Vimentin, thereby promoting cell cycle progression, migration, invasion, and Cisplatin resistance while suppressing apoptosis.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); apoptosis (inhibits); EMT (promotes); chemoresistance (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; RNA-seq; Transfection; MTS; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; TUNEL; Western Blot; Bioinformatics Analysis
Clinical significance:

Targeting hsa_circ_0096157 expression might be utilized as a new method to increase Cisplatin sensitivity and overcome NSCLC resistance to Cisplatin.
Description:

hsa_circ_0096157 is up-regulated in Cisplatin-resistant A549/DDP NSCLC cells. Functional knockdown and overexpression experiments indicate that it promotes Cisplatin resistance by enhancing proliferation, migration, invasion, and cell cycle progression while suppressing apoptosis, accompanied by regulation of cell cycle and EMT-related proteins.
Confidence score:

0.6591
Other information
Title:

Circular RNA hsa_circ_0096157 contributes to cisplatin resistance by proliferation, cell cycle progression, and suppressing apoptosis of non-small-cell lung carcinoma cells.

Journal: Molecular and cellular biochemistry
Published: 2020
PubMed ID: 32767026
Study type:

biological research
Data availability: Supplemental Table 1; Supplemental Table 2; Supplemental Fig. 1
Code availability: -